Prepare 0.8 % methyl-cellulose

Prepare 0.8 % methyl-cellulose medium as follows;

1. Add 8 g of methyl-cellulose powder into 500 ml water followed by autoclave to dissolve powder.
2. Prepare 500 ml of 2× MEM media by dissolving MEM powder in water followed by supplement with 2 % heat-inactivated FBS, 2 mM L-glutamine, 100 U/mL penicillin and streptomycin and 25 mM HEPES.
3. After filtration of 2× MEM media by 0.2 mm membrane, mix well with 500 ml of prepared methyl-cellulose and store at 4 °C.