- Place slide on stage.
- Rotate the nosepiece to the 10X objective (low power lens).
- Using the coarse adjustment, lower the nosepiece to its lowest limit.
- Using the fine adjustment control knob, bring the image of the slide into focus.
- Swing the low power lens out of position; place a drop of immersion oil on the slide; swing the oil immersion lens (100X objective) into position.
- Focus with the fine adjustment knob.
- Raise the condenser as high as it can go to improve the image contrast.
- Pull out the eyepiece.
- Look down through the barrel; close the condenser diaphragm until its edge can just be seen. The aperture of the condenser and objective are then approximately equal. This position gives the optimum optical image. Replace the eyepiece.
Cleaning the microscope
When
finished, clean the oil immersion lens with lens paper. Never touch the
lens with anything else. The lens paper can be moistened with lens
cleaner if necessary.