DNA Extraction from Mosquito (0)

1. Put 1 mosquito into an eppendorf tube with 100ul 50% w/v Chelex 100 in water.
   
2. Squish fly with pestle and place at 100C for 10 minutes.
   
3. Spin at top speed in a microfuge (>14,000xg) for 1 minute.
   
4. Transfer 10ul supernatant to new tube and add 1ul Proteinase K (0.8 units).
   
5. Incubate at 37C for 30min.
   
6. Incubate at 100C for 5 min.
   
7. Use 0.5ul as template in a 10ul PCR reaction.
   

Reagents (from Sigma):
Chelex 100 (C7901-25G) sodium form chelating resin (iminodiacetic acid)