DNA Extraction from Mosquito (2)

1. Put each mosquito in 100microl of DNAZOl (Invitrogen) and grind them.
2. centrifuge 10min at 10,000g at 15°C
3. Transfer supernatant to new tube
4. add 50microliter of 100% EtOH Mix by inverting the tubes 5-8 times and hold 5 min at room temperature.
5. centrifuge 0min at 10,000g at 15°C and remove the supernatant.
6. Wash the DNA pellet with 1.0ml of 75% EtOH and invert tube 3-6 times to mix. Centrifuge 10min at 10,000g.
7. Repeat step 6.
8. Remove the remaining EtOH and dry pellet.
9. resuspend the dry pellet with 100microliter of free DNAse Water let it overnight at 4°C to maximize pellet rehydratation.
10. Storage at -80°C